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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Development and characterization of nine highly polymorphic microsatellite primers for Platycladus orientalis
1State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Life Sciences of Beijing Normal University, Beijing, People's Republic of China.
Premise Of The Study:
Microsatellite primers were developed and characterized for the long-lived tree species Platycladus orientalis for further investigation of its population characteristics.
Methods And Results:
Nine polymorphic microsatellites were identified for P. orientalis. The number of alleles per locus ranged from four to 22 (average = 11) for 94 individual specimens examined from four populations. The observed and expected heterozygosities ranged from 0.208 to 1.000 and 0.555 to 0.931, respectively.
Conclusions:
These nine markers can be used in future studies examining population genetics and the reproductive biology of P. orientalis, thereby revealing the importance of old-growth populations for the retention of genetic diversity, and providing useful insights into the maintenance of the potential genetic resource of this species.
