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A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
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A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

Multimodal optical imaging for detecting breast cancer.

Rakesh Patel1, Ashraf Khan, Dennis Wirth

  • 1University of Massachusetts Lowell, Advanced Biophotonics Laboratory, 175 Cabot Street, Suite 110-111, Lowell, Massachusetts 01854, USA.

Journal of Biomedical Optics
|June 28, 2012
PubMed
Summary

Wide-field fluorescence polarization imaging effectively detects breast cancer during surgery. This technique accurately identifies tumor boundaries and cellular characteristics, showing promise for improved intraoperative diagnosis.

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Oncology

Background:

  • Intraoperative detection of breast cancer is crucial for effective surgical treatment.
  • Current methods for tumor margin assessment can be time-consuming and may lack accuracy.
  • Optical imaging techniques offer potential for real-time, high-resolution tissue analysis.

Purpose of the Study:

  • To evaluate multimodal optical imaging, including polarization, reflectance, and fluorescence, for intraoperative breast cancer detection.
  • To assess the utility of wide-field and high-resolution imaging for delineating tumor boundaries and cellular morphology.

Main Methods:

  • Lumpectomy specimens were stained with methylene blue (MB).
  • Acquisition of wide-field reflectance and fluorescence images across various wavelengths.
  • High-resolution confocal reflectance and fluorescence imaging.
  • Comparison of optical images with H&E histopathology for correlation analysis.

Main Results:

  • Fluorescence polarization imaging accurately identified tumor location, size, and shape in all cases.
  • Significantly higher averaged fluorescence polarization values were observed in tumor tissue compared to normal tissue.
  • Confocal fluorescence imaging provided cellular-level resolution, revealing higher polarization in individual cancer cells.

Conclusions:

  • Wide-field and high-resolution fluorescence polarization imaging demonstrates significant promise for intraoperative breast cancer delineation.
  • MB-enhanced fluorescence polarization can distinguish between cancerous and normal tissue at both macroscopic and cellular levels.
  • This multimodal optical approach may enhance surgical precision and improve patient outcomes.