Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole cross-sectional human ultrasound tomography.

Nature biomedical engineering·2026
Same author

Rotational ultrasound and photoacoustic tomography of the human body.

Nature biomedical engineering·2026
Same author

Rotational ultrasound and photoacoustic tomography of the human body.

ArXiv·2025
Same author

Whole-Body Human Ultrasound Tomography.

Research square·2024
Same author

Quantification of Cervical Elasticity During Pregnancy Based on Transvaginal Ultrasound Imaging and Stress Measurement.

IEEE transactions on bio-medical engineering·2024
Same author

Photoacoustic imaging of the dynamics of a dye-labeled IgG4 monoclonal antibody in subcutaneous tissue reveals a transient decrease in murine blood oxygenation under anesthesia.

Journal of biomedical optics·2023

Related Experiment Video

Updated: May 21, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Spectrally encoded photoacoustic microscopy using a digital mirror device.

Yu Wang1, Konstantin Maslov, Lihong V Wang

  • 1Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, 1 Brookings Drive, St. Louis, Missouri 63130, USA.

Journal of Biomedical Optics
|June 28, 2012
PubMed
Summary

We developed a new spectral photoacoustic microscopy technique using a digital mirror device for faster imaging. This method improves data acquisition speed and signal quality for optical absorption analysis.

More Related Videos

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
09:30

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Related Experiment Videos

Last Updated: May 21, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
09:30

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Published on: March 2, 2011

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Area of Science:

  • Biomedical Optics
  • Photoacoustic Imaging
  • Microscopy

Background:

  • Spectral photoacoustic microscopy (SPAM) is crucial for optical absorption imaging.
  • Traditional SPAM methods face limitations in data acquisition speed and signal-to-noise ratio (SNR).
  • Developing faster and more sensitive SPAM techniques is essential for advanced biomedical applications.

Purpose of the Study:

  • To develop and demonstrate a novel spectrally encoded photoacoustic microscopy (SE-PAM) system.
  • To enhance the data acquisition speed and SNR of SPAM.
  • To enable fast multi-wavelength tomography for optical absorption imaging.

Main Methods:

  • Utilized a digital mirror device (DMD) for external wavelength tuning and spectral encoding.
  • Multiplexed optical illumination wavelengths at a laser pulse repetition rate of approximately 2 kHz.
  • Imaged liquid samples, whole blood, and blood vessels in mouse ears.

Main Results:

  • The DMD-based external wavelength tuning significantly improved data acquisition speed compared to internal wavelength tuning.
  • Spectral encoding enhanced the SNR compared to external wavelength scanning of a wide-band laser.
  • Successfully demonstrated fast spectral imaging of optical absorption in various samples.

Conclusions:

  • The developed SE-PAM system offers a significant advancement in speed and sensitivity for photoacoustic imaging.
  • This technique enables rapid multi-wavelength tomography for detailed optical absorption analysis.
  • The findings pave the way for improved diagnostic and research capabilities in biomedical imaging.