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Updated: May 21, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Signal-on electrochemical Y or junction probe detection of nucleic acid
Zuliang Shen1, Shizuka Nakayama, Steve Semancik
1Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Abstract:
A methylene-blue (MB)-labeled molecular beacon junction probe allows for a signal-on electrochemical detection of nucleic acids via target recycling using endonucleases. Electron transfer is reduced when the MB is intercalated in the stem of the molecular beacon, but then electron transfer from MB to a gold electrode is enhanced upon cleavage of the junction probe due to increased probability of MB approaching the electrode when attached to the more flexible ssDNA.
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