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Updated: May 21, 2026

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Published on: October 16, 2015
[Determination of plasma protein binding rate of isopropylidene-shikimic acid]
Hui Kong1, Hongxia Xing, Jin Liu
1Beijing University of Chinese Medicine, Beijing 100102, China. doris7629@126.com
Objective:
To study the plasma protein binding rate of isopropylidene-shikimic acid.
Method:
The ultrafiltration was employed to determine the plasma protein binding rate of isopropylidene-shikimic acid. The plasma concentrations of isopropylidene-shikimic acid were measured by HPLC.
Result:
The plasma protein binding rate of isopropylidene-shikimic acid with dog plasma at the concentration of 0.3, 0.15 g x L(-1) and 0.5 mg x L(-1) were (4.36 +/- 0.02)%, (4.12 +/- 0.19)% and (2.23 +/- 0.59)%, respectively. While the plasma protein binding rate of isopropylidene-shikimic acid with normal human plasma at the above concentrations were (11.23 +/- 0.01)%, (10.06 +/- 0.69)% and (9.72 +/- 0.59)%, respectively.
Conclusion:
The binding rate of isopropylidene-shikimic acid with plasma protein is low.
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