Related Experiment Video
Updated: May 21, 2026
05:55
Tension-Free Weight-Bearing Model of Steroid-Induced Osteonecrosis of Femoral Head in Rats
Published on: September 27, 2024
Inhibition of mechanical stress-induced NF-κB promotes bone formation
1Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
Oral Diseases
|June 29, 2012
Summary
Mechanical force increases nuclear factor kappaB (NF-κB) activity in bone cells. This pathway influences key bone marker genes, suggesting a role for NF-κB in bone formation processes.
Area of Science:
- Cell Biology
- Biochemistry
- Orthopedics
Background:
- Nuclear factor kappaB (NF-κB) is crucial for osteogenesis.
- Understanding mechanical force's impact on NF-κB is vital for bone biology.
Purpose of the Study:
- To investigate the effects of mechanical force on NF-κB activity in osteoblast-like cells.
- To elucidate the role of NF-κB in mediating mechanical force's influence on bone marker gene expression.
Main Methods:
- U2OS cells subjected to mechanical loading.
- Western blot and electrophoretic mobility shift assay (EMSA) for NF-κB analysis.
- Real-time PCR for gene expression analysis of bone markers.
Main Results:
- Mechanical loading significantly increased nuclear P-p65 (Ser536) and total NF-κB levels.
- Ammonium pyrrolidinedithiocarbamate (PDTC) inhibited compressive force-induced NF-κB activation.
- PDTC treatment attenuated the mechanical force-induced transcriptional inhibition of alkaline phosphatase (ALP), RUNX-2, and Osteocalcin.
Conclusions:
- Mechanical force enhances NF-κB activity in osteoblast-like cells.
- Compressive force influences downstream bone marker genes via the NF-κB pathway.
Related Concept Videos
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
02:26NF-kB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:31Osteoclasts in Bone Remodeling
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
01:40Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...