Nestin immune labeling in human adult trigeminal ganglia

Nestin labels neuroepithelial stem cells and endothelial cells in newly formed blood vessels. The aim of the study was to investigate the immunolocalization of nestin in human adult trigeminal ganglia. Autopsy samples from eight human adult cadavers were paraffin embedded, and immunostained with anti-nestin antibody. Satellite glial cells (SGCs) and intraganglionic microvessels were positively labeled with nestin, which is usually expressed in endothelial cells of newly formed blood vessels. Nestin-positive SGCs have been previously reported in rat trigeminal ganglia. Our study is the first to identify them in human trigeminal ganglia. Further studies are needed to evaluate if the nestin phenotype of SGCs relates to the functional plasticity of these cells or to glial and/or neuronal remodeling in adults. Intrinsic angiogenesis in the adult trigeminal ganglion should be further checked as to whether it relates to a normal vascular remodeling or if it represents an overlooked determinant of trigeminal neuralgia.