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Simultaneous diffuse reflectance infrared determination of clavulanic acid and amoxicillin using multivariate
Aline Lima Hermes Müller1, Rochele Sogari Picoloto, Marco Flores Ferrão
1Departamento de Química, Universidade Federal de Santa Maria, Campus, 97105-900, Santa Maria, Rio Grande do Sul, Brazil.
Drug Testing and Analysis
|June 29, 2012
Summary
A new method using diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and multivariate calibration allows simultaneous determination of clavulanic acid (CA) and amoxicillin (AMO) in pharmaceuticals. This technique offers a suitable approach for quality control of these essential antibiotic components.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Pharmaceutical Analysis
Background:
- Clavulanic acid (CA) and amoxicillin (AMO) are commonly prescribed antibiotics.
- Accurate quantification of CA and AMO in pharmaceutical formulations is crucial for quality control and therapeutic efficacy.
- Existing analytical methods may require complex sample preparation or lack simultaneous determination capabilities.
Purpose of the Study:
- To develop and validate a novel method for the simultaneous determination of clavulanic acid and amoxicillin in commercial tablets.
- To evaluate the performance of diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) coupled with multivariate calibration for this application.
- To assess the suitability of the developed method for routine pharmaceutical analysis.
Main Methods:
- Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) was employed for spectral data acquisition.
- Multivariate calibration techniques, including partial least squares (PLS), interval PLS (iPLS), and synergy interval PLS (siPLS), were utilized.
- Calibration and prediction sets comprised both commercial and synthetic samples to ensure method robustness.
Main Results:
- The synergy interval PLS (siPLS) algorithm demonstrated superior performance for both analytes.
- Optimal siPLS models achieved a root mean square error of prediction (RMSEP) of 5.1 mg g(-1) for CA and 22.3 mg g(-1) for AMO.
- The developed DRIFTS-based method provided accurate and simultaneous quantification of CA and AMO.
Conclusions:
- The proposed DRIFTS method combined with multivariate calibration is a suitable and effective technique for the simultaneous determination of clavulanic acid and amoxicillin in pharmaceutical products.
- This approach offers a potentially faster and simpler alternative for quality control of antibiotic formulations.
- The method's accuracy and precision support its application in routine pharmaceutical analysis.
