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07:15
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
Published on: September 12, 2019
Poliovirus infection transiently increases COPII vesicle budding
Meg Trahey1, Hyung Suk Oh, Craig E Cameron
1Division of Biological Sciences and Center for Structural and Functional Neuroscience, The University of Montana, Missoula, Montana, USA.
Journal of Virology
|June 29, 2012
Summary
Poliovirus infection initially boosts COPII vesicle production, a key part of the host cell
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Poliovirus (PV) utilizes host cell secretory pathway membranes for viral RNA replication.
- Previous studies identified the intermediate compartment and Golgi apparatus as PV replication organelle precursors.
Purpose of the Study:
- To investigate the impact of poliovirus infection on COPII vesicle biogenesis.
- To understand the role of COPII vesicles in PV replication complex formation.
Main Methods:
- Examined the effect of PV infection on COPII vesicle formation in host cells.
- Assessed the membrane-associated levels of Sec16A, a COPII biogenesis regulator.
Main Results:
- PV infection induced a biphasic response in COPII vesicle biogenesis: early enhancement followed by inhibition.
- An increase in membrane-bound Sec16A correlated with the early rise in COPII vesicle production.
Conclusions:
- The early enhancement of COPII vesicle formation likely benefits poliovirus by providing necessary membrane precursors for replication complexes.
- Poliovirus manipulates host cell COPII vesicle dynamics to facilitate its replication cycle.
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