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Poliovirus infection transiently increases COPII vesicle budding

Meg Trahey1, Hyung Suk Oh, Craig E Cameron

  • 1Division of Biological Sciences and Center for Structural and Functional Neuroscience, The University of Montana, Missoula, Montana, USA.

Journal of Virology
|June 29, 2012
PubMed

Insights

Poliovirus infection initially boosts COPII vesicle production, a key part of the host cell

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Poliovirus (PV) utilizes host cell secretory pathway membranes for viral RNA replication.
  • Previous studies identified the intermediate compartment and Golgi apparatus as PV replication organelle precursors.

Purpose of the Study:

  • To investigate the impact of poliovirus infection on COPII vesicle biogenesis.
  • To understand the role of COPII vesicles in PV replication complex formation.

Main Methods:

  • Examined the effect of PV infection on COPII vesicle formation in host cells.
  • Assessed the membrane-associated levels of Sec16A, a COPII biogenesis regulator.

Main Results:

  • PV infection induced a biphasic response in COPII vesicle biogenesis: early enhancement followed by inhibition.
  • An increase in membrane-bound Sec16A correlated with the early rise in COPII vesicle production.

Conclusions:

  • The early enhancement of COPII vesicle formation likely benefits poliovirus by providing necessary membrane precursors for replication complexes.
  • Poliovirus manipulates host cell COPII vesicle dynamics to facilitate its replication cycle.

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