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Related Experiment Video

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3D Visualization of Retinal Vascular Pericytes in Mice by Immunostaining

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Published on: November 1, 2024

Pericytes promote selective vessel regression to regulate vascular patterning.

Nicole Simonavicius¹, Matthew Ashenden, Antoinette van Weverwijk

¹Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, 237 Fulham Rd., London, UK.

|June 29, 2012

Summary

Pericytes, via endosialin (CD248), are crucial for blood vessel regression after sprouting. Endosialin deficiency impairs this process, increasing vessel density in both normal and tumor angiogenesis.

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Area of Science:

Vascular biology
Cell biology
Developmental biology

Background:

Blood vessel networks form through sprouting angiogenesis and subsequent regression.
Pericytes stabilize vessels, but their role in regression is unclear.
Endosialin (CD248) is a pericyte receptor on new vessels.

Purpose of the Study:

Investigate pericyte roles in vascular network formation using Endosialin(-/-) mice.
Uncover novel functions of pericytes in angiogenesis.

Main Methods:

Postnatal retina angiogenesis model.
Endosialin(-/-) mouse model.
Tumor vasculature analysis.

Main Results:

Endosialin(-/-) mice show normal sprouting but defective vessel regression.
Increased vessel density observed in retina and tumors of Endosialin(-/-) mice.
Endosialin binding to basement membrane induces endothelial cell apoptosis and detachment.

Conclusions:

Pericytes, through endosialin, actively promote vessel destabilization and regression.
This function is vital in both physiological and pathological angiogenesis.
Findings impact pro- and anti-angiogenic therapy design.