Related Experiment Video
Updated: May 21, 2026
14:47
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
MyoD regulates p57kip2 expression by interacting with a distant cis-element and modifying a higher order chromatin
Anna Busanello1, Cecilia Battistelli, Mariarosaria Carbone
1Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Università di Roma La Sapienza, Viale Regina Elena 324, Roma 00161, Italy.
Nucleic Acids Research
|June 29, 2012
Summary
MyoD regulates p57kip2 gene expression via chromatin looping. This novel mechanism controls muscle development and may explain p57kip2 silencing in diseases.
Area of Science:
- Molecular Biology
- Developmental Biology
- Epigenetics
Background:
- MyoD is a key regulator of skeletal muscle differentiation.
- p57kip2 (CDK inhibitor) is crucial for coordinating muscle development and growth arrest.
- p57kip2 exhibits specific expression patterns and epigenetic imprinting, but its developmental regulation is unclear.
Purpose of the Study:
- To elucidate the regulatory mechanisms of p57kip2 expression during muscle development.
- To identify how MyoD influences p57kip2 transcription in differentiating cells.
Main Methods:
- Investigated MyoD binding to the KvDMR1 imprinting control region.
- Analyzed chromatin loop formation involving the p57kip2 promoter.
- Examined the relationship between differentiation-dependent regulation and imprinting control.
Main Results:
- MyoD binding to KvDMR1 triggers chromatin loop release, inducing p57kip2 transcription.
- Differentiation-dependent p57kip2 regulation is distinct from and subordinate to imprinting control.
- KvDMR1-mediated chromatin folding influences p57kip2's restricted developmental expression.
Conclusions:
- MyoD employs a novel mechanism involving higher-order chromatin structure modification to regulate p57kip2.
- KvDMR1-mediated chromatin folding is essential for precise p57kip2 expression during development.
- This mechanism may underlie aberrant p57kip2 silencing in certain pathologies.
Related Concept Videos
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...