Jove
Visualize
Contact Us

Related Experiment Videos

Protein phosphorylation: localization in regenerating optic axons.

D Larrivee1

  • 1Department of Physiology, Cornell University Medical College, New York, NY 10021.

Neurochemical Research
|September 1, 1990
PubMed
Summary

During goldfish optic nerve regeneration, protein phosphorylation is largely independent of synthesis in the cell body. Most major phosphoproteins are phosphorylated within the optic axons themselves.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of an ER audit to build recommendations for improving quality of care. Part I: Initial audit and recommendations.

Healthcare management forum·2001
Same author

Use of an ER audit to build recommendations for improving quality of care. Part II: Follow-up to an ER study.

Healthcare management forum·2001
Same author

Apo E allele frequencies in Alzheimer's disease, Lewy body dementia, Alzheimer's disease with cerebrovascular disease and vascular dementia.

Neuroreport·1994
Same author

[Projected trends in the number of households in Canada and the demographic impact, 1986-2011].

Cahiers quebecois de demographie·1991
Same author

Relationship between tubulin delivery and synapse formation during goldfish optic nerve regeneration.

Annals of the New York Academy of Sciences·1991
Same author

[A model for projecting a firm's manpower].

Cahiers quebecois de demographie·1988
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Neuroscience
  • Cell Biology
  • Regenerative Medicine

Background:

  • Axonal proteins undergo phosphorylation changes during goldfish optic nerve regeneration.
  • The precise location of this phosphorylation (cell body vs. axon) remains unclear.

Purpose of the Study:

  • To investigate whether axonal protein phosphorylation during optic nerve regeneration is linked to synthesis in the retinal ganglion cell body.
  • To determine if these proteins are phosphorylated within the ganglion cell axons.
  • To identify the site of phosphorylation for key proteins involved in optic nerve regeneration.

Main Methods:

  • Intraocular injection of cycloheximide to inhibit protein synthesis.
  • Intraocular injection of vincristine to block axonal transport.
  • Cranial cavity injection of 32P to label phosphoproteins in situ.

Main Results:

  • Cycloheximide significantly reduced protein synthesis but had a lesser effect on phosphorylation, indicating independence from synthesis.
  • Vincristine blocked axonal transport and protein synthesis, with phosphorylation levels remaining relatively high, suggesting axonal phosphorylation.
  • Direct injection of 32P into the cranial cavity showed higher phosphoprotein labeling in the optic tract compared to the optic nerve.

Conclusions:

  • The phosphorylation of major axonal proteins during goldfish optic nerve regeneration is largely independent of synthesis in the retinal ganglion cell body.
  • These phosphoproteins are predominantly phosphorylated within the optic axons.
  • Findings suggest that axonal compartments are critical sites for post-translational modification during nerve regeneration.

Related Experiment Videos