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Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Cells of the Epidermis01:24

Cells of the Epidermis

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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Related Experiment Video

Updated: May 20, 2026

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
06:51

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Published on: April 29, 2016

Defining an epidermal stem cell epigenetic network.

Salvador Aznar Benitah1

  • 1Center for Genomic Regulation and UPF (CRG), 08003 Barcelona, Spain. salvador.aznar-benitah@crg.es

Nature Cell Biology
|June 30, 2012
PubMed
Summary
This summary is machine-generated.

Dynamic chromatin changes in adult stem cells are crucial for self-renewal and differentiation. A genetic network of chromatin remodelers and epigenetic factors controls these genome-wide alterations in human epidermal stem cells.

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Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
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Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

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Related Experiment Videos

Last Updated: May 20, 2026

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
06:51

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Published on: April 29, 2016

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
10:03

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
08:26

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Area of Science:

  • Stem cell biology
  • Epigenetics
  • Chromatin dynamics

Background:

  • Adult stem cells require dynamic chromatin changes for self-renewal and differentiation.
  • Gene expression profiles are established through these chromatin modifications.

Purpose of the Study:

  • To investigate the genetic network controlling genome-wide chromatin changes in human epidermal stem cells.
  • To understand the role of chromatin remodelers and epigenetic factors in stem cell function.

Main Methods:

  • Analysis of chromatin remodeling pathways.
  • Epigenetic factor investigation.
  • Genome-wide studies in human epidermal stem cells.

Main Results:

  • Identification of a complex genetic network orchestrating chromatin changes.
  • Demonstration of the role of specific chromatin remodelers and epigenetic factors.
  • Characterization of genome-wide alterations in human epidermal stem cells.

Conclusions:

  • A complex genetic network of chromatin remodelers and epigenetic factors governs dynamic genome-wide chromatin changes in human epidermal stem cells.
  • These orchestrated changes are essential for maintaining stem cell self-renewal and differentiation potential.