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Related Experiment Videos

Maxi-K channels in plasma cells.

P R Brink1, E J Roemer, B Walcott

  • 1Department of Physiology and Biophysics, School of Medicine, SUNY, Stony Brook 11794.

Pflugers Archiv : European Journal of Physiology
|November 1, 1990
PubMed
Summary

Chicken Harderian gland plasma cells exhibit outward rectifying currents and maxi-K channels. These maxi-K channels are voltage and calcium sensitive, offering insights into immunoglobulin secretion.

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Area of Science:

  • Electrophysiology
  • Cell Biology
  • Immunology

Background:

  • Plasma cells are crucial for immunoglobulin secretion.
  • Understanding their electrophysiological properties is key to studying cellular function.
  • Harderian gland plasma cells offer a unique, pure preparation.

Purpose of the Study:

  • To investigate the electrophysiological properties of chicken Harderian gland plasma cells.
  • To identify ion channel types present in these cells.
  • To explore the relationship between electrophysiology and immunoglobulin secretion.

Main Methods:

  • Whole-cell patch-clamp recordings.
  • Excised inside-out and outside-out membrane patch recordings.
  • Isolation of plasma cells from chicken Harderian gland.

Main Results:

  • Whole-cell currents were dominated by slow-inactivating outward rectifying currents.
  • A single channel type, maxi-K channel, was consistently identified.
  • Maxi-K channels demonstrated voltage and calcium sensitivity, with a conductance of 200-265 pS.
  • Tetraethylammonium (TEA) reduced both whole-cell currents and single channel activity.

Conclusions:

  • Chicken Harderian gland plasma cells possess voltage- and calcium-sensitive maxi-K channels.
  • These channels influence the outward currents in plasma cells.
  • The isolated plasma cell preparation is suitable for studying electrophysiology and immunoglobulin secretion.

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