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Myelin basic protein synthesis is regulated by small non-coding RNA 715

Nina M Bauer1, Christina Moos, Jack van Horssen

  • 1Institute of Physiology and Pathophysiology, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, 55128 Mainz, Germany.

EMBO Reports
|June 30, 2012
PubMed

Insights

A novel small non-coding RNA 715 (sncRNA715) inhibits myelin basic protein (MBP) translation in oligodendrocytes. Elevated sncRNA715 levels correlate with demyelination in multiple sclerosis lesions.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • RNA Biology

Background:

  • Myelin basic protein (MBP) synthesis is crucial for central nervous system myelin formation.
  • MBP mRNA is translationally silenced during oligodendrocyte differentiation and transported until localized translation is triggered by neuronal signals.

Purpose of the Study:

  • To identify regulatory mechanisms controlling MBP translation.
  • To investigate the role of small non-coding RNAs in MBP synthesis and myelin formation.

Main Methods:

  • Identification and characterization of sncRNA715.
  • Localization studies of sncRNA715 within oligodendrocytes.
  • Association analysis of sncRNA715 with MBP mRNA transport granules.
  • Quantification of sncRNA715 levels in human multiple sclerosis lesions.

Main Results:

  • SncRNA715 was identified as an inhibitor of MBP translation.
  • SncRNA715 localizes to cytoplasmic granular structures and associates with MBP mRNA transport components.
  • Increased sncRNA715 levels were observed in demyelinated chronic human multiple sclerosis lesions.

Conclusions:

  • SncRNA715 plays a significant role in regulating MBP translation.
  • Aberrant sncRNA715 expression may contribute to demyelination in multiple sclerosis by inhibiting MBP synthesis.

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