Related Experiment Videos
Methotrexate binding to human plasma proteins
L Skibińska1, C Ramlau, J Załuski
1Department of Physical Chemistry, Karol Marcinkowski Medical Academy, Poznań, Poland.
Polish Journal of Pharmacology and Pharmacy
|March 1, 1990
Abstract:
The percentage of methotrexate (MTX) binding to plasma protein was 50.4 +/- 1.9 in healthy subject, and 32.3 +/- 3.6 in patients with cancer (breast carcinoma in most cases). Drugs used in combination with MTX in therapy (vincristine, vinblastine, cyclophosphamide, 5-fluorouracil, prednisone) depress the MTX binding to albumins. MTX binds to two kinds of albumins binding sites, with different binding constants: K1 = 8.88 mM-1 and K2 = 1.76 mM-1.