Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Making time.

Science (New York, N.Y.)·2026
Same author

Evaluating the applicability of computational super-resolution techniques for harmonic generation microscopy.

Scientific reports·2026
Same author

Clusters and Their Contributions to Ionic Conductivity in NaOTf-DME Electrolytes.

The journal of physical chemistry. B·2026
Same author

Machine Learning in Predicting the Risk of Esophagogastric Variceal Bleeding Among Patients With Liver Cirrhosis: Systematic Review and Meta-Analysis.

Journal of medical Internet research·2026
Same author

Parametrized Quantum Circuit Learning for Quantum Chemical Applications.

Journal of chemical information and modeling·2026
Same author

Nonlinear Microscopy of ECM Remodeling in Renal and Vascular Tissues: A Systematic Review Integrating Human AVF Imaging.

Medicina (Kaunas, Lithuania)·2026
Same journal

Quantum simulations of the ballistic motion of a surface adsorbate.

Physical chemistry chemical physics : PCCP·2026
Same journal

Enhancement of triplet-triplet annihilation upconversion in organically modified clay colloids.

Physical chemistry chemical physics : PCCP·2026
Same journal

What is so special about benzene? A comparison of selected carbon and silicon isomers E<sub>6</sub>H<sub>6</sub> (E = C, Si).

Physical chemistry chemical physics : PCCP·2026
Same journal

Synergistic effects of porosity and sulfur doping on hard carbon for superior sodium-ion storage.

Physical chemistry chemical physics : PCCP·2026
Same journal

Force-resolved and recurrence-based identification of dynamical heterogeneity in liquid water.

Physical chemistry chemical physics : PCCP·2026
Same journal

Thermoelectric properties of layered Bi<sub>2</sub>YO<sub>4</sub>Br: a cageless rattler host structure.

Physical chemistry chemical physics : PCCP·2026
See all related articles

Related Experiment Video

Updated: May 20, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Carotenoid based bio-compatible labels for third harmonic generation microscopy.

Danielle Tokarz1, Richard Cisek, Martyna Garbaczewska

  • 1Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North, Mississauga, Ontario L5L 1C6, Canada.

Physical Chemistry Chemical Physics : PCCP
|June 30, 2012
PubMed
Summary
This summary is machine-generated.

Carotenoids serve as effective, biologically friendly labels for third harmonic generation (THG) microscopy. This study demonstrates their nontoxic, in vivo labeling of cellular structures, enhancing imaging capabilities.

More Related Videos

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
07:53

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Related Experiment Videos

Last Updated: May 20, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
07:53

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Area of Science:

  • Nonlinear Optics
  • Biophotonics
  • Cellular Imaging

Background:

  • Third harmonic generation (THG) microscopy offers label-free imaging but often requires exogenous contrast agents.
  • Biologically friendly and nontoxic labeling agents are crucial for in vivo cellular studies.
  • Carotenoids, natural pigments, possess unique nonlinear optical properties.

Purpose of the Study:

  • To demonstrate the utility of carotenoids as biologically friendly labels for THG microscopy.
  • To characterize the nonlinear optical properties of various carotenoids for THG imaging.
  • To investigate the potential for in vivo labeling of cellular structures using carotenoids.

Main Methods:

  • Liposomes containing carotenoids were used for cell labeling via liposome-cell fusion.
  • Third-order nonlinear susceptibility (χ((3))) of carotenoids was measured using the THG ratio method.
  • Second hyperpolarizability values were extracted, considering refractive indices at fundamental and third harmonic wavelengths.

Main Results:

  • Carotenoids were characterized as THG microscopy labels (harmonophores).
  • The length dependence of second hyperpolarizability in conjugated polyenes was investigated, noting the influence of oxygen functional groups like epoxides.
  • Successful labeling of Drosophila Schneider 2 cells and myocytes was achieved using β-carotene.

Conclusions:

  • Carotenoids can be effectively used as nontoxic, biologically friendly labels for THG microscopy.
  • THG enhancement by carotenoids enables in vivo labeling of subcellular structures.
  • This approach offers a promising avenue for advanced cellular imaging techniques.