Presence of protein at the termini of intracellular adenovirus type 5 DNA

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Updated: Oct 9, 2026

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
13:36

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

P S van Wielink, N Naaktgeboren, J S Sussenbach

    Biochimica Et Biophysica Acta
    |June 20, 1979
    PubMed

    Insights

    Adenovirus type 5 DNA retains terminal protein throughout replication. This protein, found on parental, replicating, and progeny viral DNA, is likely crucial for viral DNA replication.

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Adenovirus type 5 possesses linear double-stranded DNA genomes.
    • This DNA has covalently attached proteins at its molecular ends.

    Purpose of the Study:

    • To investigate the presence and nature of terminal proteins on intracellular adenovirus type 5 DNA.
    • To determine if this protein persists during viral DNA replication.

    Main Methods:

    • Isolation of intracellular viral DNA from infected cell nuclei using 4 M guanidine hydrochloride.
    • Analysis via agarose gel electrophoresis of HsuI restriction fragments.
    • Detection of DNA-protein complexes using sucrose gradient centrifugation.

    Main Results:

    • Parental adenovirus DNA retains terminal proteins after uncoating.
    • Replicating and progeny viral DNA exist as DNA-protein complexes.
    • These complexes share properties with virion DNA-protein complexes.

    Conclusions:

    • The terminal protein on intracellular viral DNA is identical to that found in virions.
    • This terminal protein is likely involved in the replication of adenovirus type 5 DNA.

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