Pseudouridine and beta-aminoisobutyric acid excretion in urine: diagnostic and prognostic value

Area of Science:

• Biochemistry
• Urology
• Clinical Chemistry

Background:

• Biomarkers in urine, such as pseudouridine and beta-aminoisobutyric acid, are increasingly studied for diagnostic potential.
• Creatinine normalization is a standard method for correcting variations in urine concentration.
• Understanding age- and sex-related excretion patterns is crucial for establishing reference ranges and interpreting results.

Purpose of the Study:

• To investigate the sex- and age-related urinary excretion patterns of pseudouridine/creatinine and beta-aminoisobutyric acid/creatinine in healthy individuals.
• To determine if significant differences exist in the excretion ratios between adult men and women.
• To explore the potential utility of these biomarkers in diagnosing urinary tract tumors and predicting recurrence.

Main Methods:

• Quantitative analysis of urinary pseudouridine and beta-aminoisobutyric acid levels.
• Measurement of urinary creatinine for normalization.
• Statistical comparison of excretion ratios across different age groups (children vs. adults) and sexes (men vs. women).

Main Results:

• The highest urinary excretion ratios of pseudouridine/creatinine and beta-aminoisobutyric acid/creatinine were observed in the youngest children.
• No statistically significant differences were found in the excretion ratios between adult men and women.
• Excretion patterns showed distinct age-related trends.

Conclusions:

• Urinary pseudouridine/creatinine and beta-aminoisobutyric acid/creatinine excretion exhibits significant age-related variability, with highest levels in early childhood.
• These biomarkers do not show significant sex-based differences in excretion among adults.
• The findings suggest that these urinary biomarkers may serve as valuable tools for the diagnosis of urinary tract tumors and for assessing the risk of tumor recurrence.