Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Isolation of N-Fixing Bacteria from Warm-Season Pasture Grasses and the Evaluation of Nitrogen Effects on the Bacterial Communities Present in <i>Cenchrus clandestinus</i>.

Microorganisms·2026

Same author

Designed diversity: from marker-assisted backcrossing to computationally optimised polygenic introgression.

Trends in plant science·2026

Same author

Ascertainment Bias in Cattle SNP Arrays and Implications for Multibreed Genomic Predictions.

Animal genetics·2026

Same author

Negative Energy Balance in Transition Cows Induces Complex Changes in Lipid Profile of Milk.

Metabolites·2026

Same author

Factors affecting recording methane emission phenotypes of composite and crossbreed beef cattle grazing tropical and subtropical rangelands of Northern Australia.

Journal of animal science·2026

Same author

Advancing rust resistance in elite wheat with haplotype mapping and a novel introgression strategy.

Journal of experimental botany·2026

Search research articles

Related Experiment Video

Updated: May 20, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

High throughput whole rumen metagenome profiling using untargeted massively parallel sequencing.

Elizabeth M Ross¹, Peter J Moate, Carolyn R Bath

¹Biosciences Research Division, Department of Primary Industries, Bundoora, VIC 3083, Australia. elizabeth.ross@dpi.vic.gov.au

|July 4, 2012

Summary

Researchers developed a new method using massively parallel sequencing (MPS) to create rumen metagenome profiles. Rumen microbial communities were repeatable within cows, but fecal samples did not predict rumen profiles.

More Related Videos

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Related Experiment Videos

Last Updated: May 20, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Area of Science:

Microbial ecology
Metagenomics
Animal science

Background:

Rumen microorganism variation in cattle is linked to feed efficiency and methane emissions.
Untargeted massively parallel sequencing (MPS) can improve resolution in studying rumen microbial communities.
Developing a robust method for rumen metagenome profiling is crucial for understanding these variations.

Purpose of the Study:

To develop a method for generating "rumen metagenome profiles" using MPS.
To assess the repeatability of these profiles within individual cows.
To determine if rumen metagenome profiles can be predicted from fecal samples.

Main Methods:

Utilized untargeted massively parallel sequencing (MPS) data.
Developed a quantitative profiling method based on read alignment to a reference metagenome.
Compared microbial community profiles from rumen fluid and fecal samples across multiple cows.

Main Results:

Rumen metagenome profiles were highly repeatable within individual cows, irrespective of sampling location (P < 0.00001).
The repeatability estimate was 9%, with a high standard error due to the small sample size.
Fecal microbial profiles were not predictive of rumen microbial profiles; rumen and fecal communities differed significantly within the same animal.

Conclusions:

A simple, high-throughput method for metagenome profiling was successfully developed and applied.
The method enables the assessment of whole metagenome similarity using widely available freeware.
This approach is valuable for exploring and comparing complex microbial communities, particularly in the rumen.