Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Antimicrobial Proteins

Antimicrobial Proteins

Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...

Inhibitors of Bacterial Protein Synthesis

Inhibitors of Bacterial Protein Synthesis

Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...

Types of Coprecipitation

Types of Coprecipitation

Coprecipitation is the contamination of a precipitate by otherwise soluble species and occurs via different processes. In colloidal precipitates, coprecipitation occurs via surface adsorption. For instance, barium sulfate has a primary layer of adsorbed barium ions and a secondary layer of nitrate counterions. This results in contamination of the precipitate by barium nitrate.
Sometimes, ions in a crystal lattice can undergo isomorphous replacement by inclusions of similar charge and size. For...

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Randomized double-blind sham-controlled trial of thalamic versus GPi stimulation in patients with severe medically refractory Gilles de la Tourette syndrome.

Brain stimulation·2021

Same author

Stability of hepatitis E virus at high hydrostatic pressure processing.

International journal of food microbiology·2020

Same author

Stability of hepatitis E virus at different pH values.

International journal of food microbiology·2020

Same author

Selection criteria for liver transplantation in patients with hepatocellular carcinoma. Eastern and western experiences, and perspectives for the future.

Acta gastro-enterologica Belgica·2019

Same author

Prognostic relevance of topoisomerase II α and minichromosome maintenance protein 6 expression in colorectal cancer.

BMC cancer·2019

Same author

Knowledge gaps and research priorities in the prevention and control of hepatitis E virus infection.

Transboundary and emerging diseases·2018

Search research articles

Related Experiment Video

Updated: May 20, 2026

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

PCR inhibitors - occurrence, properties and removal.

C Schrader¹, A Schielke, L Ellerbroek

¹Food Hygiene and Safety Concepts, Federal Institute for Risk Assessment, Berlin, Germany.

Journal of Applied Microbiology

|July 4, 2012

Summary

Polymerase chain reaction (PCR) is a key method for detecting genetic material, but inhibitors in samples can cause false negatives. This review covers PCR inhibitor properties, occurrence, and removal strategies for accurate results.

More Related Videos

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

Published on: April 9, 2013

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Published on: December 8, 2017

Related Experiment Videos

Last Updated: May 20, 2026

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

Published on: April 9, 2013

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Published on: December 8, 2017

Area of Science:

Molecular Biology
Analytical Chemistry
Microbiology

Background:

Polymerase chain reaction (PCR) is a standard technique for detecting microorganisms and genetic markers.
PCR assays can be negatively impacted by inhibiting substances present in various sample types.
These inhibitors can reduce assay sensitivity and lead to inaccurate, false-negative results.

Purpose of the Study:

To review the general properties and diverse mechanisms of action of PCR inhibitors.
To discuss the occurrence of PCR inhibitors in specific sample matrices.
To present and evaluate strategies for removing PCR inhibitors and quality control methods.

Main Methods:

Literature review focusing on PCR inhibitor properties and sample matrices.
Analysis of various nucleic acid preparation protocols for inhibitor removal.
Discussion of quality control measures to assess inhibitor influence on PCR.

Main Results:

PCR inhibitors are a diverse group of substances with varied properties and actions.
Specific sample types often contain characteristic PCR inhibitors, requiring tailored preparation methods.
Effective removal strategies and quality control assessments are crucial for reliable PCR outcomes.

Conclusions:

Understanding PCR inhibitor characteristics is vital for optimizing detection assays.
Matrix-specific sample preparation is essential to mitigate inhibition.
Implementing robust removal and quality control strategies ensures the accuracy of PCR-based analyses.