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Updated: Aug 3, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
[Electron carriers of adrenocortical mitochondria. Terminal systems]
Abstract:
A procedure for isolation of cytochrome oxidase and cytochrome P-450 from adrenocortical mitochondria was developed. The heme and copper contents, subunit composition, optical and EPR spectra for these enzymes were determined. The effects of pH, substrates and some inhibitors on the spectra of cytochrome P-450 were studied. It was found that cytochrome oxidase did not inhibit the reactions catalyzed by cytochrome P-450; cytochrome P-450 had no inhibiting effect on the oytochrome oxidase activity.
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