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Related Experiment Video

Updated: May 20, 2026

BioMEMS: Forging New Collaborations Between Biologists and Engineers

BioMEMS: Forging New Collaborations Between Biologists and Engineers

Published on: November 1, 2007

Development and application of STEM for the biological sciences.

Alioscka A Sousa¹, Richard D Leapman

¹National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.

Ultramicroscopy

|July 4, 2012

Summary

This summary is machine-generated.

Scanning transmission electron microscopy (STEM) offers versatile biological applications. Techniques like mass mapping, nanoparticle imaging, elemental analysis, and electron tomography advance biological science research.

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Area of Science:

Biological Sciences
Microscopy
Biophysics

Background:

Scanning transmission electron microscopy (STEM) design allows efficient signal collection from electron-specimen interactions.
STEM has evolved into a versatile tool for biological research over several decades.

Purpose of the Study:

To review current applications of STEM in biological sciences.
To highlight four key areas where STEM provides significant insights.

Main Methods:

Mass determination using high-angle dark-field signals for protein assemblies.
Imaging of nanoparticles for specific biomolecule labeling.
Electron energy loss spectroscopy (EELS) for elemental mapping.
Electron tomography for 3D reconstruction of cellular structures.

Main Results:

STEM enables accurate mass determination of proteins and characterization of large assemblies.
Ultrasmall nanoparticles can be imaged for precise biological labeling.
Elemental mapping with near single-atom sensitivity is achievable.
3D reconstructions of thick cell sections with improved resolution are possible.

Conclusions:

STEM is a powerful and adaptable technique for diverse biological investigations.
Its applications range from molecular characterization to cellular imaging.
Ongoing advancements continue to expand STEM's utility in life sciences.