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Updated: May 20, 2026
08:53
Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
Published on: May 16, 2017
DOR undergoes nucleo-cytoplasmic shuttling, which involves passage through the nucleolus
Caroline Mauvezin1, Ana Sancho, Saska Ivanova
1Institute for Research in Biomedicine (IRB Barcelona), Barcelona, Spain. caroline.mauvezin@gmail.com
FEBS Letters
|July 4, 2012
Summary
The protein DOR regulates basal autophagy, a crucial cellular process for maintaining homeostasis. DOR continuously exits the nucleus, even when nucleolus integrity is disrupted, to stimulate this essential function.
Area of Science:
- Cellular Biology
- Molecular Mechanisms
- Autophagy Research
Background:
- Autophagy is primarily known as a stress-response mechanism.
- Cells require autophagy for maintaining homeostasis under basal conditions.
- Mechanisms regulating basal autophagy remain largely unknown.
Purpose of the Study:
- To investigate the role of the bi-functional protein DOR in basal autophagy.
- To elucidate the mechanisms of DOR's function in non-stress conditions.
- To understand DOR's nucleo-cytoplasmic shuttling and its impact on basal autophagy.
Main Methods:
- Studying DOR's nucleo-cytoplasmic shuttling in basal conditions.
- Analyzing the effect of DOR on basal autophagy.
- Investigating the role of the nucleolus in DOR's function.
Main Results:
- DOR regulates transcription and enhances starvation-induced autophagy.
- DOR exhibits nucleo-cytoplasmic shuttling in basal conditions.
- DOR continuously exits the nucleus and traverses the nucleolus, independent of nucleolus integrity, to stimulate basal autophagy.
Conclusions:
- DOR plays a significant role in regulating basal autophagy.
- DOR's exit from the nucleus is critical for stimulating basal autophagy.
- Nucleolus integrity is not essential for DOR's function in basal autophagy.
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