Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Thin-Layer Chromatography (TLC): Overview

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

N-Acetylcysteine in Combination with IGF-1 Enhances Neuroprotection against Proteasome Dysfunction-Induced Neurotoxicity in SH-SY5Y Cells.

Parkinson's disease·2016

Same author

Efficient regioselective acylation of quercetin using Rhizopus oryzae lipase and its potential as antioxidant.

Bioresource technology·2016

Same author

High production of erythritol from Candida sorbosivorans SSE-24 and its inhibitory effect on biofilm formation of Streptococcus mutans.

Bioresource technology·2015

Same author

Purification and characterization of a novel and robust L-asparaginase having low-glutaminase activity from Bacillus licheniformis: in vitro evaluation of anti-cancerous properties.

PloS one·2014

Same author

Eco-friendly methodology for efficient synthesis and scale-up of 2-ethylhexyl-p-methoxycinnamate using Rhizopus oryzae lipase and its biological evaluation.

Journal of industrial microbiology & biotechnology·2014

Same author

Treatment of wheat straw using tannase and white-rot fungus to improve feed utilization by ruminants.

Journal of animal science and biotechnology·2014

Search research articles

Related Experiment Video

Updated: May 20, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

A novel thin-layer chromatography method to screen 1,3-propanediol producers.

Pinki Anand¹, Rajendra Kumar Saxena

¹Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India. pinkianand@gmail.com

Journal of Industrial Microbiology & Biotechnology

|July 4, 2012

Summary

A new method screens for 1,3-propanediol producers using thin-layer chromatography and vanillin reagent. This rapid, pH-independent technique identifies microorganisms capable of producing 1,3-propanediol, a valuable industrial chemical.

More Related Videos

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Published on: July 26, 2024

Related Experiment Videos

Last Updated: May 20, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Published on: July 26, 2024

Area of Science:

Biotechnology
Microbiology
Analytical Chemistry

Background:

1,3-Propanediol is a valuable chemical with diverse industrial applications.
Current screening methods for 1,3-propanediol producing microorganisms are lacking established protocols.
Efficiently identifying and isolating these microbial producers is crucial for commercialization.

Purpose of the Study:

To develop and validate a rapid, reliable screening method for identifying bacterial producers of 1,3-propanediol.
To establish a protocol applicable to glycerol fermentation broths for microorganism selection.
To provide a foundation for harnessing the commercial potential of 1,3-propanediol biosynthesis.

Main Methods:

Utilized thin-layer chromatography (TLC) for separating compounds in bacterial glycerol fermentation broths.
Employed a vanillin reagent for colorimetric detection of 1,3-propanediol on TLC plates.
Confirmed the method's accuracy and reliability through High-Performance Liquid Chromatography (HPLC) analysis.

Main Results:

The developed TLC method successfully differentiated various compounds in fermentation broths.
A distinct purple-colored spot with a retention factor (Rf) of 0.62 specifically indicated the presence of 1,3-propanediol.
The method demonstrated effectiveness across 50 bacterial isolates and proved to be pH-independent.

Conclusions:

A novel, rapid, and pH-independent TLC-based screening method for 1,3-propanediol producers has been established.
This method offers a practical approach for identifying microorganisms with commercial potential for 1,3-propanediol production.
The validated technique facilitates the selection of superior microbial strains for industrial biotechnology applications.