Indirect prediction of surface ozone concentration by plant growth responses in East Asia using mini-open top...

We developed small and mobile open top chambers (mini-OTC) measuring 0.6 m (W) × 0.6 m (D) × 1.2 m (H) with an air duct of 0.6 m (W) × 0.23 m (D) × 1.2 m (H). The air duct can be filled with activated charcoal to blow charcoal filtered air (CF) into the chamber, as opposed to non-filtered ambient air (NF). Ozone sensitive radish Raphanus sativus cv. Red Chime and rosette pakchoi Brassica campestris var. rosularis cv. ATU171 were exposed to NF and CF in mini-OTCs at different locations in East Asia. A total of 29 exposure experiments were conducted at nine locations, Shanghai, China, Ha Noi, Vietnam, Lampang, Phitsanulok and Pathumtani, Thailand, and Hiratsuka, Kisai, Abiko and Akagi, Japan. Although no significant relationships between the mean concentrations of ambient O(3) during the experimental period and the growth responses were observed for either species, multiple linear regression analysis suggested a good relationship between the biomass responses in each species and the O(3) concentration, temperature, and relative humidity. The cumulative daily mean O(3) (ppb/day) could be indirectly predicted by NF/CF based on the dry weight ratio of biomass, mean air temperature, and relative air humidity.