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Related Experiment Video

Updated: May 20, 2026

Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy
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Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy

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Mitochondrial division in rat cardiomyocytes: an electron microscope study.

Hisashi Fujioka1, Bernard Tandler, Charles L Hoppel

  • 1Electron Microscopy Facility and Center for Mitochondrial Disease, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA. hisashi.fujioka@case.edu

Anatomical Record (Hoboken, N.J. : 2007)
|July 4, 2012
PubMed
Summary

Rat cardiomyocytes utilize two mitochondrial division methods: crista extension and membrane pinching. The specific nuclear controls dictating these distinct fission pathways remain unidentified.

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Area of Science:

  • Cell Biology
  • Mitochondrial Dynamics
  • Cardiomyocyte Function

Background:

  • Mitochondria are crucial for cardiomyocyte energy production and function.
  • Mitochondrial division (fission) is essential for maintaining a healthy mitochondrial network.
  • The mechanisms governing mitochondrial fission in cardiomyocytes are not fully understood.

Purpose of the Study:

  • To investigate the distinct mechanisms of mitochondrial division in rat cardiomyocytes.
  • To identify the morphological differences between the two observed fission processes.
  • To highlight the association of sarcoplasmic reticulum with one of the division modes.

Main Methods:

  • Morphological analysis of cardiomyocytes using advanced microscopy techniques.
  • Observation and documentation of mitochondrial division events in situ.

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Last Updated: May 20, 2026

Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy
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  • Correlation of specific cellular structures with distinct fission processes.

    • Main Results:

    • Two primary mitochondrial division pathways were identified in rat cardiomyocytes.
    • The predominant pathway involves crista extension followed by outer membrane ingrowth and scission.
    • A second pathway, termed "pinching," involves organelle narrowing and is associated with sarcoplasmic reticulum elements at constriction sites.

    Conclusions:

    • Rat cardiomyocytes employ at least two distinct mechanisms for mitochondrial division.
    • The "pinching" mode of mitochondrial fission is specifically linked to sarcoplasmic reticulum involvement.
    • The nuclear regulatory mechanisms that control the selection between these two mitochondrial division modalities are currently unknown and warrant further investigation.