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Related Experiment Video

Updated: May 20, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Separations using a porous-shell pillar array column on a capillary LC instrument.

Wim De Malsche¹, Selm De Bruyne, Jeff Op De Beeck

¹Department of Chemical Engineering, Vrije Universiteit Brussel, Brussel, Belgium. wdemalsc@vub.ac.be

Journal of Separation Science

|July 4, 2012

Summary

This summary is machine-generated.

This study demonstrates a micro-pillar array chip for liquid chromatography (LC) achieving high separation performance. The chip offers a peak capacity up to 150, nearly independent of flow rate, for protein digest analysis.

Related Experiment Videos

Last Updated: May 20, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Area of Science:

Analytical Chemistry
Separation Science
Microfluidics

Background:

Microfluidic devices offer miniaturization and enhanced separation efficiency in chromatography.
Directly interfacing microchips with conventional instruments is crucial for practical applications.

Purpose of the Study:

To evaluate the separation performance of a novel pillar array microchip interfaced with a capillary LC system.
To quantify band broadening contributions from microchip features and assess overall performance.

Main Methods:

Fabrication of a 9-cm pillar array channel (5 μm Si pillars) with mesoporous silica cladding.
Interfacing the microchip with a capillary LC instrument for gradient elution separations.
Analysis of cytochrome c and bovine serum albumin digests.

Main Results:

Estimated additional band broadening from microchip turns to be ~0.5-2 μm.
Achieved peak capacities up to 150 for protein digests.
Performance was largely independent of flow rate, indicating robustness.

Conclusions:

The pillar array microchip demonstrates excellent separation capabilities when directly interfaced with LC.
The design minimizes band broadening, enabling high peak capacity separations.
This technology holds promise for rapid and efficient biomolecule analysis.