Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid plaque neutralization test for bluetongue virus.

F C Thomas, B S Samagh

    Canadian Journal of Comparative Medicine : Revue Canadienne De Medecine Comparee
    |April 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Baseline associations between household air pollution exposure and blood pressure among pregnant women in the Household Air Pollution Intervention Network (HAPIN) multi-country randomized controlled trial.

    medRxiv : the preprint server for health sciences·2023
    Same author

    Structure and function of the antennae of Euphydryas editha (Lepidoptera: Nymphalidae).

    Journal of morphology·2018
    Same author

    A pilot study of acute phase proteins as indicators of bovine mastitis caused by different pathogens.

    Research in veterinary science·2018
    Same author

    Rupture of the rectus abdominis muscle during pregnancy.

    The Journal of obstetrics and gynaecology of the British Empire·2010
    Same author

    Hexamethonium produces both twitch and tetanic depression without fade in common African toad (Bufo regularis).

    Nigerian journal of physiological sciences : official publication of the Physiological Society of Nigeria·2010
    Same author

    Canadian regulatory requirements for recombinant fish vaccines.

    Developments in biological standardization·1997

    A novel bluetongue virus plaque neutralization test offers comparable sensitivity to traditional methods. This efficient technique saves time and reduces costs, making it ideal for widespread bluetongue virus screening and diagnostics.

    Area of Science:

    • Veterinary Virology
    • Immunology
    • Cell Culture Techniques

    Background:

    • Bluetongue virus (BTV) poses a significant threat to livestock globally.
    • Accurate and efficient diagnostic tests are crucial for controlling BTV spread.
    • Classical plaque neutralization tests can be time-consuming and resource-intensive.

    Purpose of the Study:

    • To develop and evaluate a rapid and cost-effective plaque neutralization test for bluetongue virus.
    • To assess the sensitivity of the new method compared to established techniques.
    • To explore its utility for large-scale BTV screening and dose-response analysis.

    Main Methods:

    • A modified plaque neutralization test was performed in small tissue culture wells (2.0 cm2).
    • Minimal antibody-virus contact time was employed, eliminating the need for preincubation.

    Related Experiment Videos

  • Cell culture plates were utilized as serum dilution wells, with cells added directly to the virus-antibody mixture.

    • Main Results:

    • The modified test demonstrated comparable sensitivity to classical plaque neutralization methods.
    • Significant time savings were achieved by integrating serum dilution and cell addition steps.
    • Reduced reagent costs and incubator space requirements were observed due to the small well size.

    Conclusions:

    • This optimized bluetongue virus plaque neutralization test is a sensitive and efficient alternative to traditional methods.
    • The reduced cost and time make it highly suitable for widespread application in BTV diagnostics.
    • The test can be effectively used for both screening purposes and generating dose-response data.