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Related Experiment Videos

Thiobacillus ferrooxidans, a facultative hydrogen oxidizer.

E Drobner1, H Huber, K O Stetter

  • 1Lehrstuhl für Mikrobiologie, Universität Regensburg, Federal Republic of Germany.

Applied and Environmental Microbiology
|September 1, 1990
PubMed
Summary

Thiobacillus ferrooxidans can grow using hydrogen oxidation, a newly discovered ability. This process induces hydrogenase and reduces extreme acidophilicity, with no changes in DNA homology compared to iron-oxidizing growth.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Thiobacillus ferrooxidans is known for oxidizing ferrous iron and sulfur compounds in acidic environments.
  • Its metabolic capabilities are crucial for understanding microbial roles in biogeochemical cycles.

Purpose of the Study:

  • To investigate the potential of Thiobacillus ferrooxidans to utilize hydrogen gas (H2) as an energy source.
  • To characterize the physiological and genetic adaptations associated with hydrogen oxidation in this bacterium.

Main Methods:

  • Cultivation of Thiobacillus ferrooxidans strains using H2 as the sole energy source.
  • Induction of hydrogenase enzyme activity was assessed.
  • DNA-DNA hybridization was performed to compare strains grown on H2 and ferrous iron.

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  • Comparative analysis of acidophilicity under different growth conditions.

    • Main Results:

    • Thiobacillus ferrooxidans strains, including the type strain ATCC 23270, demonstrated growth via hydrogen oxidation.
    • Hydrogenase activity was induced during cultivation on H2.
    • Strains grown on H2 exhibited reduced extreme acidophilicity compared to those grown on sulfidic ores.
    • 100% DNA homology was observed between cells grown on H2 and ferrous iron, indicating genetic similarity.
    • Hydrogen oxidation was not detected in other Thiobacillus species or Leptospirillum ferrooxidans.

    Conclusions:

    • Hydrogen oxidation represents a previously unrecognized metabolic capability of Thiobacillus ferrooxidans.
    • This finding expands the known metabolic repertoire of this important acidophilic bacterium.
    • The ability to utilize H2 may have significant implications for microbial ecology in hydrogen-rich environments.