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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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Related Experiment Video

Updated: May 20, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Note: tip enhanced Raman spectroscopy with objective scanner on opaque samples.

Mischa Nicklaus1, Christian Nauenheim, Andrey Krayev

  • 1Laboratory of Ferroelectric Nanoelectronics, INRS-EMT, 1650 Boul. Lionel-Boulet, Varennes J3X1S2 Québec, Canada. nicklaus@emt.inrs.ca

The Review of Scientific Instruments
|July 5, 2012
PubMed
Summary
This summary is machine-generated.

We achieved 14 nm resolution in tip-enhanced Raman spectroscopy (TERS) mapping of carbon nanotubes. Our novel setup enables precise laser-to-tip alignment and focus control for analyzing opaque samples.

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Last Updated: May 20, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Materials Science

Background:

  • Tip-enhanced Raman spectroscopy (TERS) offers high spatial resolution for chemical analysis.
  • Analyzing opaque samples with TERS presents challenges in sample positioning and laser focusing.
  • Previous TERS setups often lack precise control over the excitation and near-field interactions.

Purpose of the Study:

  • To demonstrate 14 nm lateral resolution in TERS mapping of carbon nanotubes.
  • To present a novel experimental setup for analyzing opaque samples using TERS.
  • To enable precise control over laser-tip alignment and focus for optimized surface plasmon excitation.

Main Methods:

  • Utilized a novel piezo-driven objective scanner for confocal side-access analysis.
  • Implemented fast and stable laser-to-tip alignment.
  • Achieved sub-wavelength precision in focus position adjustment.
  • Enabled imaging of near-field generated Raman scattering for direct control of tip enhancement.

Main Results:

  • Achieved 14 nm lateral resolution in TERS mapping of carbon nanotubes.
  • Demonstrated the capability to analyze opaque samples.
  • Validated the effectiveness of the piezo-driven objective scanner for precise alignment and focusing.
  • Showcased direct imaging of near-field Raman scattering for enhanced tip control.

Conclusions:

  • The developed experimental setup significantly advances TERS capabilities for analyzing challenging samples.
  • The novel objective scanner provides unprecedented control over excitation and near-field interactions.
  • This work paves the way for high-resolution chemical mapping of various opaque nanomaterials.