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Enthalpy of helix-coil transition of DNA: dependence on Na+ concentration and GC-content
A T Karapetian1, P O Vardevanian, M D Frank-Kamenetskii
1Kirovakan Teachers Training Institute, Armenian SSR, USSR.
Journal of Biomolecular Structure & Dynamics
|August 1, 1990
Abstract:
The enthalpy of helix-coil transition of DNA (delta H) is determined from the experiments on DNA melting with ligands by means of absolutely general formula, which contains only values directly known from the experiment (M.D. Frank-Kamenetskii, and A.T. Karapetian, Mol. Biol. USSR 6, 621 (1972)) with the combination of the "area" method (P.O. Vardevanian, et al., Biophysica 28, 130 (1983)). The experimentally obtained data show that delta H depends on both concentration of Na+ in solution and GC-content of DNA and is of high accuracy.