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Updated: May 20, 2026

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
Published on: March 4, 2018
Suhee Kim1, Cansu Agca, Yuksel Agca
1Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211, USA.
Cryopreservation and centrifugation negatively impact rat sperm function, reducing motility and membrane integrity. The cooling process significantly increases reactive oxygen species (ROS), making sperm more vulnerable to damage.
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