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Updated: May 20, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer
Sangeeta Nath1, Manli Deng, Yves Engelborghs
1Laboratory of Biomolecular Dynamics, University of Leuven, Leuven, Belgium.
Abstract:
Fluorescence correlation spectroscopy (FCS) can be used to determine the diffusion coefficient of fluorescently labeled α-synuclein. It is a technique based on the use of a confocal microscope. By applying FCS in a combination of short sampling times and repeated measurements, the disappearance of individual α-synuclein molecules (called monomers) and the formation of oligomers can be characterized during the early aggregation process.
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