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Updated: May 20, 2026

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
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Characterization of human adipose-derived stem cells.

Silvana Gaiba1, Lucimar Pereira de França, Jerônimo Pereira de França

  • 1Plastic Surgery Division, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. silvanagaiba@gmail.com

Acta Cirurgica Brasileira
|July 5, 2012
PubMed
Summary

Scientists successfully isolated and cultivated human adipose-derived stem cells (ASCs). These multipotent stem cells maintained their functional integrity and demonstrated differentiation potential for therapeutic applications.

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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Tissue engineering

Background:

  • Adipose-derived stem cells (ASCs) are multipotent cells found in adipose tissue.
  • ASCs possess significant therapeutic potential and plasticity.
  • They can differentiate into various cell lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells, and cardiomyocytes.

Purpose of the Study:

  • To isolate and cultivate human adipose-derived stem cells (ASCs).
  • To identify and characterize the isolated ASCs.
  • To assess the in vitro differentiation potential of ASCs.

Main Methods:

  • Human adipose precursor cells were obtained from subcutaneous abdominal tissue.
  • Cells were isolated using density centrifugation gradient and cultured.

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Last Updated: May 20, 2026

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  • Characterization involved phenotypic analysis, differentiation induction (adipogenic, osteogenic, chondrogenic), and flow cytometry.

    • Main Results:

    • Human ASCs replicated effectively in culture and maintained phenotype across passages.
    • Confirmed mesenchymal stem cell markers (CD73, CD90, CD105) and absence of hematopoietic markers (CD16, CD34, CD45).
    • Demonstrated successful in vitro adipogenic, osteogenic, and chondrogenic differentiation.

    Conclusions:

    • Human ASCs can be isolated and cultivated in vitro without adipogenic induction.
    • Isolated ASCs maintain functional integrity and high proliferation rates.
    • These cells exhibit multipotent differentiation capabilities, supporting their therapeutic potential.