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Updated: May 20, 2026

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Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
Published on: May 20, 2014
Monitoring the dynamics of phase separation in a polymer blend by confocal imaging and fluorescence correlation
Mikheil Doroshenko1, Maria Gonzales, Andreas Best
1Max-Planck-Institut für Polymerforschung, Mainz, Germany.
Macromolecular Rapid Communications
|July 5, 2012
Abstract:
The phase separation of the polymer blend polystyrene/poly(methyl phenyl siloxane) (PS/PMPS) is studied in situ by laser scanning confocal microscopy (LSCM) and by fluorescence correlation spectroscopy (FCS) at macroscopic and microscopic length scales, respectively. It is shown for the first time that FCS when combined with LSCM can provide independent information on the local concentration within the phase-separated domains as well as the interfacial width.

