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Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
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The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

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Related Experiment Video

Updated: May 20, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

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Published on: September 2, 2021

Conditional gene manipulation: Cre-ating a new biological era.

Jian Zhang1, Jing Zhao, Wen-jie Jiang

  • 1School of Life Science, Shandong University, Jinan, China.

Journal of Zhejiang University. Science. B
|July 5, 2012
PubMed
Summary

Site-specific recombinase (SSR) systems enable precise gene knockout, overcoming embryonic lethality. Combining SSR with inducible systems allows for temporal and tissue-specific genetic control, advancing genomic manipulation.

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Last Updated: May 20, 2026

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Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Conventional gene knockouts often cause embryonic lethality, limiting research.
  • Site-specific recombinase (SSR) systems offer a solution for conditional gene manipulation.
  • Inducible gene expression systems provide temporal control over genetic modifications.

Purpose of the Study:

  • To review the advancements and applications of site-specific recombinase (SSR) systems in conditional gene targeting.
  • To highlight the integration of SSR systems with inducible expression for precise genomic manipulation.
  • To explore novel applications of SSR systems in disease modeling and functional genomics.

Main Methods:

  • Utilizing Cre-loxP, Flp-FRT, and ΦC31 SSR systems for targeted gene modification.
  • Combining SSR systems with tetracycline and tamoxifen-inducible gene expression.
  • Developing conditional gene trapping, multipurpose alleles, and gene silencing strategies.

Main Results:

  • SSR systems enable tissue-specific gene knockout, circumventing embryonic lethality.
  • Inducible SSR systems allow for stage-specific gene knockout and transgenic expression.
  • SSR systems facilitate the creation of precise disease models with mutations and chromosomal abnormalities.

Conclusions:

  • The integration of SSR and inducible systems offers powerful tools for advanced genomic manipulation.
  • These conditional tools expand the possibilities for studying gene function and creating disease models.
  • Genomic manipulation is entering a new era with the sophisticated control offered by SSR systems.