Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic monitoring of antibody drug conjugates targeting TROP2 or HER2 in breast cancer using circulating tumor cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Inducing radiation resilience in frozen animal cells via mRNA coding for tardigrade damage-suppressor protein in support of space travel and Lunar storage.

The Journal of heredity·2026
Same author

Author Correction: HER2 expression identifies dynamic functional states within circulating breast cancer cells.

Nature·2026
Same author

Reduction in Red Blood Cell Lysis by Polymer Intervention During Rodent Liver Normothermic Machine Perfusion.

Transplantation direct·2026
Same author

Oscillatory flow for contactless particle trapping.

Lab on a chip·2026
Same author

Microfluidic automation improves oocyte recovery from follicular fluid of patients undergoing in vitro fertilization.

Nature medicine·2026

Related Experiment Video

Updated: May 20, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Nanoporous micro-element arrays for particle interception in microfluidic cell separation.

Grace D Chen1, Fabio Fachin, Elena Colombini

  • 1BioMEMS Resource Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.

Lab on a Chip
|July 6, 2012
PubMed
Summary

Ultra-high porosity nanoporous micro-posts significantly enhance cell capture in microfluidic devices. These novel structures improve particle interception efficiency, leading to greater cell isolation success for bacteria and cancer cells.

More Related Videos

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Related Experiment Videos

Last Updated: May 20, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Area of Science:

  • Biotechnology
  • Materials Science
  • Microfluidics

Background:

  • Microfluidic immunoaffinity cell capture faces challenges with cell-surface interaction control.
  • Solid capture surfaces in microfluidics suffer from flow stagnation, hindering cell convection.
  • Achieving specific cell binding requires overcoming limitations of current microfluidic systems.

Purpose of the Study:

  • To investigate the use of ultra-high porosity nanoporous micro-posts for enhancing particle interception efficiency in microfluidic channels.
  • To compare the performance of nanoporous posts against solid posts for cell capture.
  • To validate the effectiveness of nanoporous microfluidic devices for cell isolation.

Main Methods:

  • Utilized both computational modeling and experimental validation.
  • Fabricated and tested nanoporous vertically aligned carbon nanotube (VACNT) post arrays.
  • Compared VACNT posts with solid polydimethylsiloxane (PDMS) posts of identical geometry.
  • Employed bacteria (∼1 μm) and cancer cell lines (∼15 μm) as model systems.

Main Results:

  • Nanoporous posts enhance particle interception via increased direct interception and reduced near-surface hydrodynamic resistance.
  • Capture efficiency increased by 6-fold for bacteria and 4-fold for cancer cells compared to solid posts.
  • Demonstrated improved cell capture efficiency using nanoporous VACNT arrays over solid PDMS arrays.

Conclusions:

  • Ultra-high porosity nanoporous micro-posts offer a significant advancement for microfluidic cell isolation.
  • The developed platform represents a new generation of microfluidic devices for enhanced cell capture.
  • Nanoporous structures effectively overcome limitations of solid surfaces in microfluidic cell capture systems.