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Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
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Self-assembled mannan nanogel: cytocompatibility and cell localization.

Sílvia A Ferreira1, Vera Carvalho, Carla Costa

  • 1Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.

Journal of Biomedical Nanotechnology
|July 7, 2012
PubMed
Summary
This summary is machine-generated.

Amphiphilic mannan nanogels self-assemble in water, showing no cytotoxicity and potential for macrophage-targeted drug delivery. These nanogels are stable, spherical, and internalized by mouse bone marrow-derived macrophages (BMDM).

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Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
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Published on: November 25, 2017

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery

Background:

  • Mannan, a polysaccharide, can be modified to create amphiphilic properties.
  • Self-assembly of amphiphilic polymers in aqueous media forms nanostructures with potential biomedical applications.

Purpose of the Study:

  • To synthesize and characterize amphiphilic mannan nanogels.
  • To evaluate the in vitro cytocompatibility and cellular uptake of these nanogels by macrophages.
  • To assess the potential of mannan nanogels as nanomedicines for targeted delivery.

Main Methods:

  • Synthesis of amphiphilic mannan via Michael addition.
  • Characterization of nanogel physicochemical properties (size, charge).
  • In vitro cytotoxicity assays (MTT, LDH, Live/Dead) on 3T3 fibroblasts and BMDM.
  • Comet assay for DNA damage assessment.
  • Confocal laser scanning microscopy for cellular uptake studies.

Main Results:

  • Amphiphilic mannan nanogels are stable, spherical, polydisperse (50-140 nm), with a near-neutral negative surface charge.
  • No significant cytotoxicity was observed up to 0.4 mg/mL in 3T3 cells and BMDM.
  • Comet assays showed no DNA damage in fibroblasts but potential damage in BMDM.
  • BMDM internalized the nanogels via endocytosis and exocytosis.

Conclusions:

  • Self-assembled mannan nanogels exhibit favorable physicochemical properties and in vitro cytocompatibility.
  • The observed cellular uptake by BMDM suggests potential for macrophage-targeted delivery.
  • Mannan nanogels represent a promising nanosystem for nanomedicine applications, including vaccine and drug delivery for disease treatment.