Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Classification of Titrimetric Analysis Based on Reaction Types01:01

Classification of Titrimetric Analysis Based on Reaction Types

Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
Titrations between an acid and a base lead to neutralization reactions that form...
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026
Same author

Simultaneous Probe of the Charm and Bottom Quark Yukawa Couplings Using tt[over ¯]H Events.

Physical review letters·2026
Same author

Measurement of WWZ and ZH Production Cross Sections at sqrt[s]=13 and 13.6 TeV.

Physical review letters·2025

Related Experiment Video

Updated: May 20, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Ultrio and Ultrio Plus non-discriminating reactives: false reactives or not?

R Charlewood1, P Flanagan

  • 1New Zealand Blood Service, Auckland, New Zealand. richard.charlewood@nzblood.co.nz

Vox Sanguinis
|July 7, 2012
PubMed
Summary

Detecting occult hepatitis B virus (HBV) DNA in blood donations is challenging. Analysis of routine nucleic acid testing (NAT) showed that some donations with low HBV DNA levels may appear reactive, requiring careful donor assessment.

More Related Videos

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
12:19

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Published on: April 22, 2022

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
05:52

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

Related Experiment Videos

Last Updated: May 20, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
12:19

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Published on: April 22, 2022

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
05:52

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

Area of Science:

  • Blood Transfusion Safety
  • Virology
  • Molecular Diagnostics

Background:

  • Occult hepatitis B infection presents challenges for detection due to low and fluctuating viral DNA levels.
  • Nucleic acid testing (NAT) is crucial for identifying infectious agents in donated blood.
  • Accurate detection of hepatitis B virus (HBV) is vital for preventing transfusion-transmitted infections.

Purpose of the Study:

  • To analyze the performance of Ultrio and Ultrio Plus NAT assays in detecting HBV in New Zealand blood donations over four years.
  • To evaluate the significance of discriminatory-negative, anti-HBc-reactive donations in the context of HBV screening.
  • To assess the implications of assay performance on blood donor selection and transfusion safety.

Main Methods:

  • Retrospective analysis of four years of routine blood donation testing data in New Zealand.
  • Utilized Ultrio and Ultrio Plus nucleic acid testing (NAT) assays for multiplex viral screening.
  • Correlated multiplex assay reactivity with discriminatory assays and mandatory serological markers (anti-HIV, anti-HCV, HBsAg) and anti-HBc testing.

Main Results:

  • 0.09% of donations showed multiplex assay reactivity but were negative in discriminatory and mandatory serological assays.
  • These donations were significantly more likely to be anti-hepatitis B core (anti-HBc) reactive.
  • A substantial proportion of anti-HBc-reactive, discriminatory-negative donations showed repeat reactivity or reactivity in alternate NAT assays, suggesting low-level viremia.

Conclusions:

  • Hepatitis B virus DNA levels in some donors may be near the limit of detection for current NAT assays.
  • False reactivity should not be presumed for donors failing discriminatory assays; careful evaluation is necessary.
  • Caution is advised when determining the acceptance of donations from donors with discriminatory-negative, anti-HBc-reactive results to maintain blood safety.