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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
13:32

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

High quality genomic DNA extraction from postmortem fetal tissue.

S Addison1, N J Sebire, A M Taylor

  • 1Academic Neonatology, Institute for Women's Health, University College London, London, UK.

The Journal of Maternal-Fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
|July 7, 2012
PubMed
Summary

High-quality genomic deoxyribonucleic acid (DNA) can be extracted from fetal tissues, even after several days postmortem. Muscle tissue yielded the highest quality DNA for genomic testing.

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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Published on: August 6, 2009

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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
18:17

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Published on: August 6, 2009

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Postmortem tissue analysis is crucial for various scientific investigations.
  • Assessing the quality and yield of genomic deoxyribonucleic acid (DNA) from fetal tissues is essential for downstream applications.
  • Understanding the impact of postmortem interval on DNA integrity is critical.

Purpose of the Study:

  • To evaluate the yield and quality of genomic DNA extracted from diverse postmortem fetal tissues.
  • To determine the suitability of extracted DNA for genomic testing.
  • To investigate the influence of postmortem interval on DNA yield and quality.

Main Methods:

  • Genomic DNA extraction from fetal tissues collected during autopsy.
  • Assessment of DNA yield and quality using ultraviolet spectrometry and agarose gel electrophoresis.
  • Polymerase chain reaction (PCR) to confirm DNA integrity for genomic analysis.

Main Results:

  • Non-degraded genomic DNA was successfully extracted from all tested fetal tissues.
  • Liver tissue yielded the lowest quality DNA, while muscle tissue provided the highest quality.
  • The postmortem interval did not significantly affect DNA yield or purity.

Conclusions:

  • High-quality genomic DNA is obtainable from fetal muscle tissue, even after extended postmortem periods.
  • Fetal muscle is a suitable source for genomic DNA extraction in postmortem investigations.
  • The findings support the use of fetal tissues for reliable genomic analysis.