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Updated: May 20, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

Second-generation sequencing for gene discovery in the Brassicaceae.

Alice Hayward¹, Guru Vighnesh, Christina Delay

¹ARC Centre of Excellence for Integrative Legume Research, School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia.

Plant Biotechnology Journal

|July 7, 2012

Summary

The Brassicaceae family conserves genes (NSP1 and NSP2) typically used in plant-microbe symbioses, despite lacking these associations. This finding aids in understanding gene function in diverse crops.

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Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

Area of Science:

Plant molecular biology
Genomics
Crop science

Background:

Brassicaceae crops are agriculturally vital but lack symbiotic associations with mycorrhizal fungi for nutrient uptake.
Genes for symbiosis, like NSP1 and NSP2, were acquired by legumes for interactions with rhizobia bacteria.

Purpose of the Study:

To investigate the conservation of symbiosis-related genes (NSP1 and NSP2) in the Brassicaceae family.
To demonstrate the utility of second-generation sequencing (SGS) for gene discovery in complex crop genomes.
To explore potential alternative functions of these conserved genes in Brassicaceae.

Main Methods:

Applied second-generation sequencing (SGS) to identify gene homologs in Brassicaceae.
Utilized bioinformatics tools for gene analysis in polyploid crop genomes.
Conducted comparative genomics to assess gene conservation.

Main Results:

Identified conserved NSP1 (Nodulation Signalling Pathway 1) and NSP2 genes in diverse Brassicaceae species.
Demonstrated SGS data's effectiveness for discovering gene homologs in genomes with limited sequence information.
Showcased the application of SGS data to improve reverse genetics analyses in crops.

Conclusions:

NSP1 and NSP2 genes are conserved in Brassicaceae despite the absence of mycorrhizal and rhizobial symbioses.
SGS is a valuable tool for gene discovery and analysis in complex plant genomes.
Hypothesize that Brassica NSP genes may be involved in other root-microbe interactions, repurposed for symbioses during evolution.