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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
05:35

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Published on: January 17, 2020

Chromium speciation in environmental samples using a solid phase spectrophotometric method.

Alaa S Amin1, Mohammed A Kassem

  • 1Chemistry Department, Faculty of Science, Benha University, Benha, Egypt.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|July 7, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a new solid phase extraction method for chromium speciation in natural waters. The technique enhances sensitivity for accurate chromium analysis in various environmental and food samples.

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Spectroscopy

Background:

  • Chromium speciation is crucial for environmental and health risk assessment.
  • Existing methods for chromium analysis can be complex and lack sensitivity.
  • A need exists for efficient preconcentration and speciation techniques for trace chromium.

Purpose of the Study:

  • To develop and validate a solid phase extraction (SPE) method for chromium speciation.
  • To determine chromium(III) and total chromium in natural water samples.
  • To apply the method for preconcentration of total chromium in diverse sample matrices.

Main Methods:

  • Solid phase extraction using dextran-type anion-exchange gel (Sephadex DEAE A-25).
  • Spectrophotometric determination of chromium after complexation with 4-(2-benzothiazolylazo)2,2'-biphenyldiol (BTABD).
  • Reduction of Cr(VI) to Cr(III) for total chromium determination; Cr(VI) calculated by difference.

Main Results:

  • High molar absorptivities (2.11×10^7 and 3.90×10^7 L mol⁻¹cm⁻¹) achieved.
  • Linear calibration range of 0.05-1.45 μg L⁻¹ with low RSD (<1.85%).
  • Low detection and quantification limits (e.g., 8.0 and 27 ng L⁻¹ for 1000 ml sample).

Conclusions:

  • The proposed SPE-spectrophotometric method is sensitive and reliable for chromium speciation.
  • The method effectively preconcentrates chromium in natural waters and digested solid samples.
  • Results show good agreement with the electrothermal atomic absorption spectrometry (ET AAS) method.