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An immunoassay for the determination of total IgA subclass antibodies in human serum
An enzyme immunoassay capable of determining total IgA1 and IgA2 concentrations in human serum has been developed. Subclass-specific monoclonal antibodies are bound to polyacrylamide bead-conjugated anti-mouse immunoglobulin antibodies. Bound IgA is detected with an anti-IgA peroxidase conjugate and the standard curve is linear in the region 0.25 - 2.0 micrograms/ml. Coefficient of variation values range from 0.24 - 5.77% for the IgA1 standard curve and from 0.86 - 5.92% for the IgA2 standard curve. Inter-assay variation for the IgA1 and IgA2 control sample values were 8.2% and 13.4%, respectively.
An enzyme immunoassay capable of determining total IgA1 and IgA2 concentrations in human serum has been developed. Subclass-specific monoclonal antibodies are bound to polyacrylamide bead-conjugated anti-mouse immunoglobulin antibodies. Bound IgA is detected with an anti-IgA peroxidase conjugate and the standard curve is linear in the region 0.25 - 2.0 micrograms/ml. Coefficient of variation values range from 0.24 - 5.77% for the IgA1 standard curve and from 0.86 - 5.92% for the IgA2 standard curve. Inter-assay variation for the IgA1 and IgA2 control sample values were 8.2% and 13.4%, respectively.