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A Myb transcription factor of Phytophthora sojae, regulated by MAP kinase PsSAK1, is required for zoospore

Meng Zhang1, Jing Lu, Kai Tao

  • 1Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Ministry of Education, Nanjing, China.

Plos One
|July 7, 2012
PubMed

Insights

Phytophthora sojae mitogen-activated protein kinase PsSAK1 regulates zoospore development through the PsMYB1 transcription factor. Silencing PsSAK1 impairs zoospore formation and plant infection, highlighting PsMYB1

Area of Science:

  • Plant pathology
  • Molecular biology
  • Fungal genetics

Background:

  • Phytophthora sojae mitogen-activated protein kinase (MAPK) PsSAK1 is crucial for host infection and zoospore viability.
  • The downstream regulatory mechanisms of PsSAK1 are not fully understood.

Purpose of the Study:

  • To investigate the downstream targets and functions of PsSAK1 in Phytophthora sojae.
  • To identify key genes regulated by PsSAK1 during infection and zoospore development.

Main Methods:

  • 3'-tag digital gene expression (DGE) profiling of PsSAK1-silenced mutants.
  • Quantitative real-time PCR (qRT-PCR) to validate gene expression.
  • Phenotypic analysis of PsMYB1-silenced mutants.

Main Results:

  • PsSAK1 silencing altered the expression of several Myb family transcription factors, including PsMYB1.
  • PsMYB1 expression is regulated by PsSAK1 and is essential for zoospore formation and release.
  • PsMYB1 is required for Phytophthora sojae's ability to infect soybean plants via zoospore-mediated processes.

Conclusions:

  • The R2R3-type transcription factor PsMYB1 acts downstream of the MAP kinase PsSAK1.
  • PsMYB1 is indispensable for normal zoospore development and the infectivity of Phytophthora sojae.

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