Related Experiment Video
Updated: May 20, 2026
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
Published on: May 6, 2019
[Sulfatide-loaded CD1d tetramer to detect typeII NKT cells in mice]
Gu-qin Zhang1, Han-xiang Nie, Jiong Yang
1Department of Respiratory Diseases, Zhongnan Hospital, Wuhan University, China. zgqxiao@126.com
Aim:
To create a method of detecting typeII natural killer T (NKT) cells of mice.
Methods:
Biotinylated mouse CD1d monomers were mixed with sulfatide at a molar ratio of 1:3 (protein:lipid) and incubated at room temperature overnight, and then 80 μg of streptavidin-PE was added into 200 μg of the CD1d-sulfatide mixture and incubated at room temperature for 4 h to get sulfatide/CD1d tetramer. Flow cytometry was used to detect the percentage of typeII NKT cells in mononuclear cells (MNCs) of lung and spleen of normal mice, as well as the percentage of typeII NKT cells in spleen MNCs of mice after stimulated with sulfatide.
Results:
In normal mice, the percentage of typeII NKT cells accounted for (0.875±0.096)% and (1.175±0.263)% in MNCs of spleen and lung; the percentage in spleen MNCs after activated with sulfatide was (2.75±0.603)%, which significantly increased as compared with that in normal mice (P<0.01).
Conclusion:
Sulfatide-loaded CD1d tetramer is an effective method of detecting typeII NKT cells in mice.
