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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

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Related Experiment Video

Updated: May 10, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

U1 snRNA rewrites the "script".

Evan C Merkhofer, Tracy L Johnson

    Cell
    |July 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

    A splicing factor controls messenger RNA (mRNA) length by influencing the polyadenylation machinery. This discovery is crucial for understanding gene expression in active brain and immune cells.

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    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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    Related Experiment Videos

    Last Updated: May 10, 2026

    Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
    11:34

    Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

    Published on: August 9, 2019

    Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
    07:35

    Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

    Published on: August 6, 2019

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
    08:53

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

    Published on: September 15, 2021

    Area of Science:

    • Molecular Biology
    • Gene Expression Regulation
    • RNA Processing

    Background:

    • Eukaryotic messenger RNA (mRNA) expression relies on numerous RNA processing factors.
    • The precise regulation of mRNA length is critical for cellular function and gene expression.
    • Neuronal and immune cells exhibit complex gene expression patterns, particularly upon activation.

    Discussion:

    • The study investigates the interplay between pre-mRNA splicing and polyadenylation.
    • A specific splicing factor's role in modulating polyadenylation efficiency is elucidated.
    • This mechanism directly impacts the final length of mRNA molecules.

    Key Insights:

    • A pre-mRNA splicing factor actively regulates the polyadenylation machinery.
    • This regulation determines mRNA transcript length, influencing protein-coding potential.
    • The findings have significant implications for isoform expression in activated neuronal and immune cells.

    Outlook:

    • Further research can explore the specific splicing factors involved in this cross-talk.
    • Understanding this mechanism could lead to new therapeutic targets for diseases involving aberrant gene expression.
    • Investigating the cell-type specific roles of this regulatory pathway is warranted.