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Staphylococcus pseudintermedius colonization patterns and strain diversity in healthy dogs: a cross-sectional and
Narayan Chandra Paul1, Sofia Cathrine Bärgman, Arshnee Moodley
1Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark. napa@life.ku.dk
Veterinary Microbiology
|July 10, 2012
Summary
Staphylococcus pseudintermedius colonization is common in healthy dogs, particularly on the perineum and in the mouth. This study reveals high strain diversity, suggesting frequent transmission within the dog population.
Area of Science:
- Veterinary Microbiology
- Canine Health
- Bacterial Genomics
Background:
- Staphylococcus pseudintermedius is a key bacterium in canine pyoderma.
- Understanding its carriage and diversity in healthy dogs is crucial for infection control.
- Limited longitudinal data exists on S. pseudintermedius colonization in canines.
Purpose of the Study:
- To investigate the prevalence and diversity of Staphylococcus pseudintermedius colonization in healthy dogs.
- To characterize the spatial and temporal distribution of S. pseudintermedius strains.
- To compare S. pseudintermedius carriage in dogs with Staphylococcus aureus carriage in humans.
Main Methods:
- Cross-sectional and longitudinal sampling of nasal, oral, perineal, and inguinal sites in 119 healthy dogs.
- Bacterial identification using Polymerase Chain Reaction (PCR).
- Strain typing via pulsed field gel electrophoresis (PFGE) for high-resolution genetic diversity analysis.
Main Results:
- S. pseudintermedius was isolated from 69% of dogs, with the perineum (66%) and mouth (65%) being the most common carriage sites.
- PFGE analysis demonstrated significant genetic heterogeneity among isolates from the same dog.
- Longitudinal sampling confirmed persistent carriage in most dogs, with some initially missed in cross-sectional screening.
Conclusions:
- Canine carriage of S. pseudintermedius is more frequent and genetically diverse than human S. aureus carriage.
- Dog-specific factors like hygiene and social behavior likely promote bacterial transmission.
- Further research into transmission dynamics and host-pathogen interactions is warranted.
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