Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cholesterol: Significance and Regulation

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...

Bone Disorders

Bone Disorders

Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...

Bone Remodeling

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gene-body DNA methylation of ONECUT2 predicts its expression and prostate cancer aggressiveness in needle biopsies.

Biomarker research·2026

Same author

Drug Development.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Same author

DNA Binding by BosR Controls RpoS-Dependent and -Independent Gene Expression in Borrelia burgdorferi.

Molecular microbiology·2025

Same author

ONECUT2: a validated drug target and lineage plasticity driver in prostate cancer and other malignancies.

Endocrine-related cancer·2025

Same author

Comparison of QuPath and HALO Platforms for Analysis of the Tumor Microenvironment in Prostate Cancer.

Laboratory investigation; a journal of technical methods and pathology·2025

Same author

ONECUT2 reprograms neuroendocrine fate and is an actionable therapeutic target in small cell lung cancer.

Molecular medicine (Cambridge, Mass.)·2025

Search research articles

Related Experiment Video

Updated: May 20, 2026

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

Hypercholesterolemia promotes an osteoporotic phenotype.

Kristine Pelton¹, Jaclynn Krieder, Danese Joiner

¹Department of Orthopaedic Surgery, Children's Hospital Boston, Massachusetts 02115, USA.

The American Journal of Pathology

|July 10, 2012

Summary

High cholesterol levels (hypercholesterolemia) lead to bone loss and weakened bones in mice. This study suggests a link between cholesterol imbalance and osteoporosis development, impacting bone mineral density and strength.

More Related Videos

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Published on: September 15, 2018

Related Experiment Videos

Last Updated: May 20, 2026

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Published on: September 15, 2018

Area of Science:

Bone Biology
Metabolic Diseases
Osteoporosis Research

Background:

Emerging evidence suggests a connection between hypercholesterolemia and osteoporosis.
Direct evidence linking cholesterol homeostasis to bone health remains limited.

Purpose of the Study:

To investigate the direct impact of hypercholesterolemia on bone health.
To establish a preclinical model for studying cholesterol's effects on bone.

Main Methods:

Utilized isocaloric high-fat/high-cholesterol and low-fat/no-cholesterol diets in two mouse strains over 4 months.
Employed micro computed tomography (micro-CT) for bone mineral density assessment.
Conducted mechanical testing on femurs and serologic/histomorphologic analyses.

Main Results:

Hypercholesterolemic mice exhibited significant loss of cortical and trabecular bone in femurs and vertebrae.
Reduced bone mineral density was observed in vertebrae (average ≈90 mg/mL decrease) and calvariae (average ≈60 mg/mL decrease) in one strain.
Mechanical integrity of femurs was compromised, showing decreased failure load (≈10 N decrease) and energy to failure.
Analyses indicated hypercholesterolemia promotes osteoclastogenesis.

Conclusions:

Hypercholesterolemia contributes to bone loss and negatively impacts bone mechanical properties.
These findings support a role for hypercholesterolemia in osteoporosis development.
The study provides a valuable model for testing interventions against cholesterol-induced bone damage.