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Updated: May 20, 2026

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
Published on: August 9, 2024
Characterizing the neurite outgrowth inhibitory effect of Mani
Manisha Mishra1, Sachiko Lee, Michelle K Lin
1School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
Abstract:
Mani (myelin-associated neurite-outgrowth inhibitor) protein is implicated in both axonal guidance and axonal regeneration after central nervous system (CNS) injury. Here, we applied a neurite outgrowth assay, coupled with a siRNA-driven investigation and immunocytochemistry, to unveil Mani's axonal outgrowth inhibitory effect in embryonic rat cortical primary neurons in vitro. We further demonstrate Mani's neuronal localization in comparison with a principal subunit, Cdc27, of the anaphase promoting complex (APC). Considering the protein structure of Mani obtained via a series of bio-computational studies, we propose a Cdc27-Mani-APC-related signalling pathway may be involved in CNS axon regeneration.
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